Embryo Service
To safeguard particularly valuable lines, we offer an all-round service. Cryopreservation of embryos or sperm and embryo transfer as well as in vitro fertilization are the core points of a safeguarding and sanitation strategy, which can be completed by health monitoring, isolation and, if possible, therapy.
Cryopreservation allows safe long-term storage of valuable strains.
Various variants are possible in the field of biotechniques. Find out about the numerous possibilities in the Biotechniques data sheet. Choose the variant that is suitable for you and contact us regarding the procedure and prices.
mfd Diagnostics offers several options of biotechniques for mice. These include:
Superovulation
- Superovulation of mice incl. plug check and documentation
- Delivery of the plug-positive animals by mfd Diagnostics or by an external courier
Generation of embryos in the 2-cell stage
(embryo transfer is performed by the customer)
- Delivery of the 2-cell embryos by mfd Diagnostics or by an external courier
Creation of embryos in the 2-cell stage including cryopreservation of the embryos
(revitalization and embryo transfer is carried out by the customer)
- Delivery of the cryopreserved embryos by mfd Diagnostics or by an external courier
- Transport in liquid nitrogen
Or
- Interim storage of cryopreserved embryos in liquid nitrogen at mfd Diagnostics until delivery
Mouse embryo transfer
- Embryo transfer and delivery of at least 2 visibly pregnant recipient females.
Or
- Delivery of rederived offspring incl. health certificate
Cryopreservation: Collection and freezing of mouse embryos and mouse spermatozoa.
- Cryopreservation of 150-300 embryos or 10 vials of spermatozoa.
- In vitro and in vivo vitality test
- Storage at mfd Diagnostics
Mouse revitalization
- Thawing of cryopreserved embryos, embryo transfer and delivery of at least 2 visibly pregnant females.
- Thawing of cryopreserved embryos, embryo transfer and delivery of offspring incl. health certificate
- Mouse sperm revitalization via IVF and delivery of offspring incl. health certificate
Line rescue: restoration of a breeding colony when it no longer reproduces due to its age or phenotype.
- Mouse sperm collection and revitalization via IVF.
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