Autofluorescence Analyzer

NADHJA® lab 355

mfd Diagnostics NADHJA
  • compact laboratory Fluorescence Analyzer
  • UV stimulation at 355 nm
  • robust housing / IP 54
  • 7" touch screen colour display
  • fanless

NADHJA® lab 355

is a high-quality, innovative laboratory device. It allows the analysis of the autofluorescence of the metabolic enzyme NAD (nicotinamide adenine dinucleotide) present in the reduced form in a very precisely defined tissue volume after excitation by UV laser pulses. The fluorescence excitation is achieved by the nanosecond pulse of a frequency-tripled Nd:YAG laser at a wavelength of 355 nm. The detection of the fluorescence is achieved via a photomultiplier and signal amplifier. The use of special fibre optic probes allows a wide range of applications.

Technical data
analytical wavelength (440 ± 35 ) nm
analytical frequency 0,01 – 100 Hz
detector Hamamatsu PMT H10720
laser Nd:YAG, 355 nm, 1,5 µJ, fibre coupled
pulse width 1,1 ns
fibre entrance/-exit 2x FSMA
µController ARM Cortex-M4F
operating system Real Time Engineers Ltd. FreeRTOS V8.1.2
data interface USB 2.0
display 7,0“ TFT WVGA Touchscreen
Laserklasse 1 Laser class 1
Medicine technology
mfd Diagnostics NADHJA
News

Wendelsheim, 21th of November, 2017


NADHJA® Autofluorescence-Analyzer


Measurement of NADH concentration changes in tissue


With the NADHJA®-Analyzer it is now possible to measure NADH continuously, invasively or noninvasively, in real time and by laser induction of the photon emission (autofluorescence) of the NADH.


The analysis is performed by the excitation of NADH autofluorescence by means of defined laser pulses in the nanosecond range. The selective absorption of NADH in the UV-A range is used. For the measurement no separate fluorescence marker is necessary. The fluorescence pulses are evaluated by a special pulse amplifier which separates the NADH fluorescence from the background fluorescence.


Advantages:

  • autofluorescence
  • real time
  • flexible application
  • can be used locally
  • animal-friendly

Applications:

  • analysis/measurement of NADH concentration in the cell metabolism
  • monitoring of cellular oxygen demand
  • demand-oriented oxygen monitoring
  • analysis of cell activity in oxygen deficiency states
  • detection of cell pathological disorders of cell metabolism

Research areas:

  • metabolic research
  • cancer research
  • cardiovascular diseases
  • diabetes research
  • neurological diseases
  • in vitro monitoring of cell vitality
  • pharmacology / toxicology

You will find detailed information in our folder.

Arrange an appointment with us for a demonstration of the device.